

CONTENTS. 

 PART I. GENERAL TECHNIQUE. 



CHAP. PAGE 



I. Sterilization. 



Introduction, - 3 



Section I. Sterilization by dry heat, 4 



(1) Sterilization in a naked flame, p. 4. (2) Sterilization by hot air, 

 p. 4. 



Section II. Sterilization by moist heat, - 7 



(1) Sterilization in steam at 100 C., p. 7. (2) Sterilization in 

 steam under pressure, p. 9. (3) Sterilization by discontinuous 

 heating, p. 12. 



Section III. Sterilization by filtration, - 14 



(1) Filtration of water, p. 15. (2) Filtration of culture media, 

 p. 18. (3) The filtration of small quantities of liquid, p. 24. 



Section IV. Sterilization by antiseptics, 26 



II. Culture media. 



Introduction, - 28 



Section I. Liquid media, 30 



(1) Media made from animal tissues and fluids, p. 30. (2) Media 

 made from vegetable tissues, p. 37. (3) Synthetic media, p. 38. 



Section II. Solid media, - 39 



(1) Gelatin media, p. 39. (2) Agar media, p. 42. (3) Media made 

 from albuminous fluids and tissues, p. 45. (4) Vegetable media, 

 p. 55. (5) Coloured media, p. 56. 



III. Incubators. 



Introduction, - 58 



Section I. Devices for automatically regulating the temperature 



of incubators, - 59 



Section II. Incubators heated by coal gas, 61 



Section III. Incubators heated by electricity, 65 



Section IV. Incubators heated by petrol, gasoline, or petro- 

 leum oil, 66 



