190 POST MORTEM EXAMINATIONS 



These solutions ought to be prepared just before use. The use of Flemming's 

 solution should be limited to the hardening of nerve tissues, and the pieces 

 should be very small. Many stains cannot be used after Flemming's solu- 

 tion ; the best to use are haematoxylin, safranin and the basic aniline dyes. 



Suspend the tissue in the solution and leave it for 36-72 hours (weak solu- 

 tion) or 1-24 hours (strong solution), wash in running water for 24 hours, 

 transfer to distilled water for 1 hour, and then for 24 hours to each of the 

 following solutions successively, viz. 70 per cent., 80 per cent., 90 per cent, 

 alcohol. 



5. Flemming-perchloride solution. A mixture of acid perchloride and 

 Flemming's solution combines the advantages of both. The mixture is 

 prepared according to the following formula : 



Saturated aqueous solution of perchloride of mercury, - 500 c.c. 



1 per cent, aqueous solution of chromic acid, - - 500 



Osmic acid crystals 1 gram. 



Glacial acetic acid, - 50 c.c 



Harden for 1224 hours. Wash and transfer to alcohol as in the case of 

 Flemming's solution. 



