

INOCULATION OF GUINEA-PIGS 299 



material was inoculated in the inguinal region. The guinea-pig killed five days 

 later showed a local thickening only. The ten-day guinea-pig showed in 

 addition to a local lesion, lesions in the superficial inguinal glands and in the 

 axillary and sternal glands. The twenty-day animal showed extension of 

 the disease to the deep inguinal, iliac and manubrial glands and to the spleen, 

 liver and portal glands : one tubercle was found in the lung. In the thirty- 

 day guinea-pig the disease had reached the lungs and bronchial glands, 

 the intestines and mesenteric glands as well as the cervical, lumbar and 

 coeliac glands. The thirty-eight day guinea-pig showed tubercles in the 

 kidneys. 



[The duration of life of the guinea-pig will depend upon the dose of tubercle 

 bacilli administered : but the extent of the disease is not found to vary much, 

 since an extremely small number of either bovine or human tubercle bacilli 

 is able to set up general progressive tuberculosis in the guinea-pig (English 

 Commission).] 



2. Cutaneous inoculation. If the inguinal region of a guinea-pig be shaved 

 and rubbed with a piece of absorbent wool soaked in sputum containing 

 tubercle bacilli, the corresponding glands will become enlarged a week or 

 fortnight later and the animal will die of tuberculosis in about two months. 

 Post mortem, lesions typical of the disease will be found (Osman Nouri). 

 This method of inoculation is very useful for diagnosis, because it involves 

 no risk of death from septicaemia, an accident very likely to happen if the 

 material be inoculated sub-cutaneously. 



3. Intra-peritoneal inoculation. The course of the disease is similar to 

 that just described but is more rapid. Death occurs in 2-6 weeks being 

 preceded by an increasing degree of cachexia. Lesions similar to those 

 already described are found in the 



tissues : the peritoneum is infiltrated ^ j*W: "'*** 



with tubercles and the omentum forms 



a compact, caseous mass, while the 



mesenteric and inguinal glands are also 



caseous. The indurated nodule at the 



site of inoculation (chancre) is, of course, 



non-existent. * * * /B 



A large dose of an human or avian w \ /$? ^ .-^ \ 

 culture is fatal to guinea-pigs in a few ^ i* A r~&\ V 

 days when inoculated intra-peritoneally. (Ifc^ $ 9 



Post mortem there is an excess of fluid v 

 in the pleurae but no tubercles are visible ** 



in the internal organs (Koch, Straus and * A 



Gamaleia). " 



[Following intra-peritoneal inocula- 

 finn flio nrmrao r>f fTi rliPQap i a FlG - 19 - Scraping from the spleen of a 



tion tne course ol tne disease is as tuberculous g U i ne a-pig (carboi-fuchsin and 



follows : in guinea-pigs which die in methylene blue). (Oc. 2, obj. Ath, Zeiss.) 

 under 14 days, there is a local lesion 



in the wall of the abdomen : the omentum is thickened, the mesentery and 

 peritoneum are inflamed and covered with a thin membrane, the mesentery 

 is also thickened : the spleen is enlarged and speckled with minute points, 

 the liver shows minute foci, the kidneys are normal : the pleural cavities 

 are filled with fluid, the lungs are collapsed and often consolidated and show 

 minute grey points in the dark red areas : the pyloric, lumbar and ventral 

 mediastinal (or sternal) glands are severely affected, the portal and other 

 abdominal lymphatic glands less affected while the bronchial glands are 

 usually only slightly affected. In guinea-pigs which survive for 3 weeks to a 



j & Si f% 2? 9 



.""^*' ;Y; . 



* ^ f ^p. 4. 





