INOCULATION OF CATTLE 



303 



2. Intra-peritoneal inoculation. Death occurs 2-3 months after inocula- 

 tion of a pure culture of tubercle bacilli into the peritoneal cavity. Inoculation 

 is followed by peritonitis with excess of fluid, the formation of false membranes, 

 adhesion of the coils of the intestine, and infection of the glands. The disease, 

 ultimately, becomes generalized. [Intraperitoneal inoculation with moderate 

 doses of cultures of either the human or the bovine tubercle bacillus is usually 

 but not invariably fatal (work of the English Commission).] 



3. Intra- venous inoculation. Death takes place 1-2 months after inocula- 

 tion into a vein of O25 c.c. of a thick emulsion of bacilli from a glycerin-agar 

 culture. The pulmonary lesions are the most marked while the liver, spleen, 

 etc. may also show tubercles. 



4. Inhalation. Tappeiner infected dogs by causing them to breathe an 

 atmosphere charged with dried and powdered tuberculous sputum. Lesions 

 were found, post mortem, in the lungs, spleen and kidneys. 



5. Infection by feeding. Arloing fed dogs with cultures of the tubercle 

 bacillus and in three out of seven cases found lesions in the alimentary canal ; 

 in two other cases the disease was generalized (in the spleen and lungs). 

 [Dogs are very resistant to infection with tuberculosis by feeding, especially 

 adult animals (A. S. Griffith and F. Griffith, for the English Commission).] 



D. Cattle. 



(a) Cattle are very susceptible to infection with the bacillus of the bovine type. 



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Fm. 193. Section of the udder of a cow which died 299 days after intra- 

 mammary inoculation of bacilli derived from a typical bovine virus. The 

 specimen is taken from an affected portion of the mammary tissue showing 

 early infection Note (1) The high vascularity, (2) the interstitial infiltration 

 with bacilli, (3) the entrance of bacilli into the glandular epithelium, and (4) the 

 excretion of bacilli into a mammary tubule. It is of importance to note that 

 lesions such as this, which are obviously unrecognizable clinically, excrete 

 numerous bacilli. Carbol-fuchsin and methylene blue, x 400. (Eastwood.) 

 (See footnote p. 295.) 



In calves, infection by way of the alimentary canal leads to very severe 

 symptoms (Vallee). [" Feeding with the bovine tubercle bacillus does not 



