

MORPHOLOGY 311 



The section may be treated with orange-yellow which stains particularly the red 

 cells of the blood. After staining with orange, dehydrate in absolute alcohol. 

 5. Clear in clove oil and xylol. Mount in balsam. 



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FIG. 197. Section, of human tuberculous lung. Carbol-fuchsin and methy- 

 lene blue. (Oc. 2, obj. / 2 th, Zeiss.) 



3. Ehrlich's method. 



1. Stain in aniline-violet for 12 hours in the cold. 



2. Decolourize in 33 per cent, nitric acid for a few seconds. Wash. 



3. Complete the decolonization in absolute alcohol. 



4. Counterstain with a saturated aqueous solution of vesuvin. 



5. Dehydrate rapidly in absolute alcohol. Clear with clove oil and xylol. 

 Mount in balsam. 



4. Letulle's method. 



1. Stain the nuclei with haematoxylin as in Kiihne's method. Wash in distilled 

 water. 



2. Stain with Ziehl's fuchsin in the cold for a quarter of an hour. Wash rapidly 

 in distilled water. 



3. Wash in absolute alcohol for 30 seconds. 



4. Treat with the following solution for 5 minutes : 



lodgriin, ----- 1 gram. 



2 per cent, solution of carbolic acid, - - 100 c.c. 



5. Decolourize in absolute alcohol. 



6. Clear in clove oil and xylol. Mount in balsam. 



The groundwork is stained very pale grey-lilac ; the nuclei, violet ; the bacilli, 

 deep red. This method can be used for tissues hardened in Mailer's fluid. 



5. Herman's method. 



Herman's method (p. 309) can, according to its author, be applied to the staining 

 of frozen sections of tissues fixed in acetic perchloride solution. The technique is 

 the same as for films, the stain being allowed to act for 1 minute with steam rising. 



6. Lustgarten's method (modified). 



1. Stain for some hours in the cold in carbol-fuchsin. 



2, 3, 4, 5. As for films (p. 310). 



6. Wash in water. Dehydrate rapidly in absolute alcohol. 



7. Clear in clove oil and xylol. Mount in balsam. 



This method is useful when searching for bacilli in sections of the liver, where 

 they are often difficult to find. It is also available for tissues hardened in Miiller's 

 fluid. 



