414 IDENTIFICATION OF THE TYPHOID BACILLUS 



METHOD OF DIAGNOSIS. 



COLON BACILLUS. 



TYPHOID BACILLUS. 



4. Growth in milk. 



5. Growth on potato. 



6. Action on neutral-red. 



7. Growth on artichoke. 



Growth on the syn- 

 thetic media of Ncegeli, 

 Remy and Sugg, 

 Frankel and others. 



9. Growth 

 water. 



in peptone 



10. Single colonies on de- 

 colourized fuchsin-agar 

 (Endo). 



11. Growth on (a) Caffe- 

 ine media. (6) Malach- 

 ite green. 



12. Flagella. 



13. Action of anti-typhoid 

 serum (using the serum 

 in its highest agglutinat- 

 ing dilution). 



14. Serum of a guinea-pig 

 immunized with the 

 organism. 



15. Simultaneous inocula- 

 tion of anti-typhoid 

 serum. 



16. Complement fixation. 



Coagulation in 24 

 hours. 1 



36 



Thick brownish growth 

 (inconstant). 



Reduced. 



Abundant growth, the 

 medium becoming 

 green (inconstant). 



Copious and rapid growth 

 (inconstant). 



Indol. 



Red colonies. 



No growth (possible ex- 

 ceptions). 



Flagella short and few 

 in number (3 to 4 on 

 each bacillus). 



No agglutination. 



The serum does not ag- 

 glutinate a true typhoid 

 bacillus in a dilution of 

 1 in 40. 



If the bacillus is virulent 

 the simultaneous inoc- 

 ulation of antityphoid 

 serum does not pro- 

 tect the animal. 



No deviation of comple- 

 ment with a heated 

 antityphoid serum. 



No coagulation. 



Thin colourless glazed 

 growth (inconstant). 



Not reduced. 



No apparent growth. 

 No change in colour of 

 medium. 



A poor growth appear- 

 ing slowly (inconstant). 



No indol. 



Colourless colonies. 



Growth (possible excep- 

 tions). 



Numerous (8-18), long, 

 wavy, undulating fla- 

 gella. 



Distinct agglutination 

 (possible exceptions). 2 



The serum agglutinates 

 a true typhoid bacillus 

 in a dilution of 1 in 40 

 (some possible excep- 

 tions). 



If the bacillus is viru- 

 lent, the simultaneous 

 inoculation of anti- 

 typhoid serum pro- 

 tects the animal. 



Complement is deviated 

 with a heated anti- 

 typhoid serum. 



1 Should no coagulation occur sow the bacillus in a shallow layer of milk. Some 

 strains of the colon bacillus only coagulate milk under these conditions. 



2 Typhoid bacilli recently isolated from the body occasionally fail to agglutinate until 

 they have been sub-cultured several times in broth. 



