THE BACILLUS OF RHINOSCLEROMA 419 



Cultures. The bacillus of rhinoscleroma grows on all the ordinary labora- 

 tory media. Unlike the pneumobacillus it does not grow on slightly acid 

 media, and does not ferment carbohydrates ; further, its cultures are much 

 more scanty than those of the pneumobacillus (Paltauf). 



In cultures, the bacillus of rhinoscleroma always forms capsules which may 

 be easily demonstrated by /Hie following method. Dilute a little of the 

 growth in a 1 per cent, solution of acetic acid, spread on a slide, dry, and 

 stain with aniline-violet : examine in water. 



On broth, agar and serum The growth on these media is very similar to 

 the growth of the pneumobacillus but more scanty. 



On gelatin the growth is thread-like and very limited. The tylotate 

 appearance so characteristic of the pneumobacillus is never produced. 



On milk. The medium is not coagulated. 



Experimental inoculation. Laboratory animals are not susceptible to 

 inoculation with the bacillus of rhinoscleroma. 



Lowenberg's bacillus. 



(The bacillus of ozcena.) 



The bacillus found in the mucous exudates in ozaena by Lowenberg and Abel 

 is no longer regarded as the cause of ozsena. It resembles the pneumobacillus so 

 closely that it seems necessary to regard the two organisms as identical (Viollet, 

 de Simoni, and others). 



The microscopical appearance, the cultural characteristics and the results of inocula- 

 tion are the same in both cases. The only differences between them seem to be that 

 the bacillus of ozsena does not ferment all the carbohydrates which are fermented 

 by the pneumobacillus and does not coagulate milk. 



