642 THE GONOCOCCUS 



to these observers was of therapeutic value in gonorrhoeal rheumatism and 

 gonorrhoeal infections of the geni to-urinary tract in man. 



The serum of guinea-pigs and rabbits immunized by intra-peritoneal 

 inoculations of cultures of the gonococcus agglutinates old cultures of the 

 gonococcus and also, but to a less extent, young cultures. 



Bruckner and Christeanu after inoculating a horse on several occasions with 

 cultures of the gonococcus obtained a powerfully agglutinating serum. This 

 serum exhibited therapeutic properties against an intra-peritoneal inoculation 

 in rabbits. 



The serum of a person suffering from gonorrhoeal prostatitis agglutinated 

 an old culture but not a young culture (Wildbolz). 



Normal human serum and normal guinea-pig serum did not possess agglu- 

 tinating properties. 



[Human vaccine therapy. Vaccines prepared by Wright's method (p. 605) 

 give distinctly encouraging results in the treatment of gonorrhoea and its 

 complications. An autogenous vaccine must, of course, be used to obtain 

 the most satisfactory results but while this is in preparation a " stock " 

 vaccine prepared from a laboratory strain may be administered to avoid 

 delay in the treatment. The initial dose should consist of five or ten million 

 organisms and the amount inoculated on subsequent occasions must be 

 determined by the extent of the local inflammation and by the degree of 

 general malaise following each dose. It is important that a severe reaction 

 be not excited.] 



SECTION IV.-DETECTION AND ISOLATION OF THE GONOCOCCUS. 



Pus from the urethra and from the various secondary suppurations should 

 form the material for examination. 



In collecting pus from the urethra, after cleansing the meatus squeeze 

 the penis from the root to the glans, and aspirate the pus into a pipette or 

 collect it on a platinum loop. 



' -* n 4 



*i 



FIG. 303. Gonococcus in pus from the urethra. Gram's stain and dilute 

 carbol-fuchsin. (Oc. 2, obj. T l 2 th, Zeiss.) 



(a) Microscopical examination. In making film preparations the cover- 

 glasses should not be pressed together too firmly and should, be slid apart 

 gently so that the pus cells are not broken : if the films be treated roughly 



