ASPERGILLUS GLAUCUS 695 



temperature of growth varies from 15-37 C. according to the species. The 

 best media to use are : diluted beer-wort, Raulin's liquid medium (p. 38), 

 milk, gooseberry- juice, peptone-broth containing sugar and glycerin, potato, 

 moist bread, and agar or gelatin made with beer-wort or Raulin's medium, etc. 



These fungi may be isolated on plates of gelatin or agar made with Raulin's 

 medium. The method described for the isolation of the Mucoracidse is also 

 applicable. Cultures in cells can be prepared as described on p. 675. 



3. Experimental inoculation. The pathogenicity of the different species 

 varies considerably : the amount of disease produced depends also upon the 

 number of spores inoculated. Birds are the most susceptible of all animals 

 to the inoculation of parasites of this genus, then come rabbits, guinea-pigs 

 and monkeys. It is best to inoculate the material directly into a vein but 

 infection also follows intra-peritoneal, sub-cutaneous and other forms of 

 inoculation. The lesions differ according to the species inoculated but 

 generally speaking partake of the nature of a pseudo-tuberculosis. 



SECTION I. THE GENUS ASPERGILLUS. 



The genus Aspergillus is characterized by non-septate spore-bearing hyphse 

 swollen at the tip. The swollen end is covered with short branches or sterig- 

 mata each terminating in a row of conidia (fig. 330). The arrangement of 

 the conidial apparatus resembles the inflorescence of an onion. 



1. Aspergillus glaucus. 

 Syn. Aspergillus herhariorum. 

 This fungus is very widely distributed in 

 nature. It is often seen as green spots on 

 decomposing organic matter. It does not 

 appear to be pathogenic though some observers 

 think they have found it in birds : possibly 

 in those cases the fungus was mistaken for a 

 variety of Aspergillus fumigatus (Pinoy). It 

 grows at low temperatures, but cannot be 

 cultivated in the warm incubator (37 C.). The 

 spores are large (8-15//. in diameter). 



2. Aspergillus repens. 



This species is very closely related to the 



foregoing from which it is distinguished mainly FIQ 3so ^ Aspergillus gUucus , 

 by the smaller size of its spores (4r-8fj- in 

 diameter). It has been found in the wax which sometimes accumulates in the 

 external auditory meatus (Siebenmann). It does not appear to have any 

 pathogenic property. 



3. Aspergillus malignus. 



This species was found by Lindt in the ear of a man. It is pathogenic 

 for rabbits. Growth takes place at 35-37 C. 



The swelling on the conidial hyphse is pear-shaped and not, as in the two 

 preceding species, spherical, and for two-thirds of its area is covered by 

 undivided sterigmata carrying chains or rows of conidia greenish-white in 

 colour and measuring 3-4/x in diameter. 



4. Aspergillus fumigatus. 



Laulanie has shown that Aspergillus fumigatus is capable of producing a 

 condition of pseudo-tuberculosis when inoculated experimentally into animals. 



