9 6 



LECTURES ON IMMUNITY 



Madsen and Walbum have also investigated the influ- 

 ence of the temperature on this process, which in this case 

 is peculiarly low, p being found to be 10,500 only. As 

 a comparison we may cite the saponification of ethyl- 

 acetate, where JJL has a value of the same order of magni- 

 tude, namely 11,160. They investigated the reaction 

 velocity of a solution containing 4 c.c. of the solution 

 of tetanolysin, 0.08 c.c. of a 2 per cent solution of Witte's 

 pepton, and 3.9 c.c. of physiological salt-solution at 37.1, 

 31.2, 27.5, and 17.8 C. respectively. 



They found the following figures : 



INFLUENCE OF THE TEMPERATURE ON THE DESTRUCTION OF TETANOLYSIN 



BY PEPTON 



The agreement between the observed and the calculated 

 values is very satisfying; even at 17.8 C., where the devi- 

 ations are the greatest, they still fall within the possible 

 errors of observation. 



The calculated constants (K) of reaction, according to 

 the bimolecular formula, are given in the following table 

 O= 10,240): 



