VELOCITY OF REACTION. HETEROGENEOUS SYSTEMS 1 05 



The first figures indicate an activity known to be too 

 low (too long time). This may be due to errors in the 

 measuring of the time. It is supposed that the liquids are 

 immediately brought to the temperature of the thermostat 

 in which they are placed. Evidently this is not quite true, 

 and therefore in a more exact calculation a certain time 

 should be subtracted from the observed one. 



Henri has carried out some interesting experiments on 

 the haemolysis of chicken erythrocytes by means of normal 

 dog-serum. He added different quantities of the serum 

 to 30 c.c. of a 10 per cent suspension of the erythrocytes 

 and so much of 0.9 per cent NaCl solution as to bring 

 the whole volume to 40 c.c. Then he observed that the 

 haemolysis proceeded at first rapidly, later on more slowly, 

 until it reached a limit value. This value was found to 

 be: 



Quantity of serum in c.c. (<j) 0.3 0.4 0.5 0.75 I 1.5 

 Limit of haemolysis in per cent 15 19.5 30 56 93 100 



93 & l S-3 23.5 33 60 93 (100) 



The limit value increases more rapidly than the first 

 power of the quantity of serum, but not as much as the 

 square of it; the last figures indicate that the limit is 

 nearly proportional to the power f of q. 



To illustrate the progress of haemolysis with time Henri 

 gives the following figures : 



Quantity of serum 



in c.c 0.15 0.2 0.3 0.4 0.5 0.75 I 1.5 2 



Haemolysis in per 

 cent after 12 min. 8.5 19.1 30 



(8.5) (19.1) (34) 

 Haemolysis in per 



cent after 36 min. 5.0 6.9 10.0 28.2 66.6 95.6 

 (4.5) (8.0) (12.5) (28.1) (50) (100) 



