io6 



LECTURES ON IMMUNITY 



Haemolysis in per 



cent after 76 min. 4.1 8.4 13.0 19.5 47.0 78.5 98.3 100 



(3-i) (7-0) (12.5) (19.5) (43.9) (78) (100) (100) 

 Haemolysis in per 



cent after 107 min. 3.3 5.5 11.7 15.7 23.6 50.0 85.0 100 100 



(2.0) (3.6) (8.0) (14.3) (22.3) (50.0) (89.0) (100) (loo) 

 Haemolysis in per 



cent after 200 min. 4.8 7.9 14.4 18.3 29.0 55.0 90.0 100 100 



(2.4) (4.3) (9.6) (17.1) (26.7) (60) (ico) (100) (100) 



In the brackets are written the figures which are pro- 

 portional to the squares of the quantities of haemolysing 

 serum. As will be seen from these, the agreement with 

 the observed figures is fairly good (within the errors of 

 observation), if the time lies below 76 minutes (in most 

 cases the time of reaction was about one hour in similar 

 experiments). For longer times of reaction the action of 

 small quantities is somewhat greater than the rule of the 

 square root demands. 



Henri 1 found that the progress with time follows very 

 closely the law of monomolecular reactions. He mixed 

 30 c.c. of a 10 per cent suspension of chicken erythro- 

 cytes with 9.5 c.c. of 0.8 per cent sodium chloride solu- 

 tion and the following quantities of dog-serum. The 

 haemolysis, x> is given with its limit value as unit. The 

 reaction-constants are calculated from the formula 



^ l ^ l 



K =-log . 



/ & i x 



1 Victor Henri: C. r. de la Societe Biologique, 58. 36-39 (Jan. 4, 1605). 



