126 



LECTURES ON IMMUNITY 



emulsion was held at constant temperature and at given 

 times samples were removed and titrated for their free 

 acid. (The acid initially added was subtracted.) The 

 first figures concern an emulsion in o. I n. sulphuric acid, 

 the later figures are found for emulsions in o.i and 0.4 n. 

 acetic acid. 



The constants are 1.47 for the first, 180 and 380 respec- 

 tively for the later experiments. 



The deviation of the figures for the later times seems to 

 indicate that we have here to do with either real equilibria 

 in the presence of lipases acting on ethylic butyrate, mono- 

 glycerid of butyric acid, and triacetin, and known from 

 Kastle and Loewenhart's, Hanriot's, and A. E. Taylor's in- 

 vestigations, 1 or some perhaps false equilibrium. These are 

 very common with enzymes, as Tammann (I.e.) found. 

 Even the figures for less than 45 minutes indicate a devia- 

 tion from the premises of the calculation. There are also 



1 Kastle and Loewenhart : Amer. Chem. Journ., 24. 491 (1900) ; Hanriot, 

 C. R. t 132. 212 (1901); A. E. Taylor: Journ. Biol. Chemistry, 2. 87 (1906). 



