NEUTRALISATION OF H^EMOLYSINS 



169 



ment is valid also for the action of such hsemolysins as 

 tetanolysin. It seems as if also in this case a chemical 

 binding takes place, but the compound seems to be to a 

 higher degree dissociated, so that the limit is not so sharp 

 as for the alkalies. 



To give an idea of this process I reproduce here some 

 figures for the haemolytic action of taurocholate of so- 

 dium, saponin, potassium hydrate, and solanin. For potas- 

 sium hydrate 2.5 per cent suspensions of bovine corpuscles, 

 for the other haemolysins 2 per cent suspensions of equine 

 erythrocytes, were used. Following the addition of the 

 suspension of cells to the poison, the mixture was shaken 

 and then for one hour placed in an incubator of 37 C. and 

 after this eighteen hours in a refrigerator and then com- 

 pared with the solutions of haemoglobin of different concen- 

 trations prepared by the haemolysis of different quantities 

 of the same blood-corpuscles by pure water. The concen- 

 tration is given for the potassium hydrate in fractions of a 

 normal solution, for the other substances in fractions of 

 the whole fluid (weight of dissolved substance : weight of 

 solution). 



