188 



LECTURES ON IMMUNITY 



NEUTRALISATION OF TETANOLYSIN BY nc.c. io~ 6 n CHOLESTERIN 

 AT 37 C. (MADSEN AND WALBUM) 



If we choose a concentration in which 5 c.c. of the 

 tetanus broth are contained in loc.c. as the unit concen- 

 tration, we find a value 0.02*1 for K in the equation : 

 (Cone, of tetanolysin) x (cone, of cholesterin)= 



K (cone, of compound). 



Even in this case the observed values of T for high values 

 of n are a little less than the calculated ones. 



Even some so-called neutral substances exert an influ- 

 ence upon the haemolytic action of tetanolysin. The pres- 

 ence of salts in large doses increases the action. In this 

 case the erythrocytes are suspended in a physiological 

 solution of cane sugar. If to this so much salt is added 

 that the solution is 0.04 normal, the haemolysis (during i 

 hour at 37 C.) increases to about the double value. A 

 o.oi normal solution is without appreciable influence. 

 Different salts in the same concentration seem to exert the 

 same influence. This influence is probably due to an ac- 

 celeration of the velocity of reaction. For this reason 

 solutions of lysin seem to be more poisonous in a physio- 

 logical salt-solution than in one containing cane sugar. 



