NEUTRALISATION OF H^EMOLYSINS 



IQI 



afterward calculated the results. 1 Preliminary experiments 

 showed that the effect increased with the quantity of anti- 

 toxin present and that the dilution of the reacting sub- 

 stances exerted no influence, so that the prevailing chemical 

 process must be a monomolecular one. 



One c.c. of a bouillon containing tetanolysin, was added to 

 0.8 c.c. of a solution containing antilysin of which 0.18 c.c. 

 were equivalent to I c.c. of the solution containing the lysin. 

 This mixture was kept at 37 C. for a certain time, /, and 

 afterward mixed with 3 c.c. of the poison and the whole 

 held at 37 C. during 30 minutes. The toxicity, q, was de- 

 termined in the ordinary way and found to increase with 

 the time, /, so that q converged against a maximum value 

 G^. If q 9 valid for the time o is taken as a unit, the dif- 

 ference q q Q is a measure of the effect investigated. The 

 difference q^ q indicates the progress of the effect with 

 time. This quantity is treated as corresponding to a mono- 

 molecular process with the constant of reaction K. The 

 results are given in the following table : 



THE PROGRESS OF DANYSZ'S EFFECT FOR TETANOLYSIN WITH TIME 



1 Madsen and Arrhenius : Medd. fr. Vet. Ak : s. Nobelinstitut, 1. No. 3 

 (1906). 



