THE COMPOUND H^MOLYSINS 249 



at 37 C. before the erythrocytes and the immune-body 

 were added. 



EXPERIMENTS OF MORGENROTH AND SACHS ON ANTIALEXIN 



i. SHEEP BLOOD ERYTHROCYTES 



Immune-body, from goat treated with sheep erythrocytes ; alexin, normal serum of 



guinea-pigs ; antialexin, immune-serum from goat, treated with rabbit-serum 

 a 6 c c : b c \ a. 



0.3 0.006 0.35 58 1.2 



0.05 0.006 O.I 17 2 



o.oi o.oi o -75 7-5 7-5 



0.005 -5 0-015 -3 I0 



2. Like I, but the antialexin is from a rabbit which has been treated with guinea- 

 pig-serum 



a. b c c : b c : a 



0.2 0.0035 0.005 I -4 0.025 



o.i 0.025 -4 J -6 0.4 



3. BOVINE ERYTHROCYTES 



Immune-body, from rabbit treated with bovine erythrocytes; alexin, normal serum 

 of guinea-pigs ; antialexin, from goat, treated with rabbit-serum 



a. b c c:b c : a 



0.2 0.05 0.75 15 38 



0.004 o- 1 -i i.o 25 



4. ERYTHROCYTES FROM HUMAN BLOOD 



Immune-body, from a rabbit, treated with human erythrocytes; alexin, normal 

 serum from a rabbit ; antialexin, from goat, treated with rabbit-serum 



a b c c '. b c '. a 



0.2 0.05 0.075 x -5 -3 8 



o.i 0.05 -35 -7 0.35 



0.05 o.i 0.025 o>2 5 -5 



Only in the case 2 is there the approximate proportionality 

 between c and b which was expected by the authors. Evi- 

 dently if the alexin is bound by the antialexin without 

 dissociation, the quantity of antialexin necessary for neu- 

 tralisation will be proportional to the quantity of alexin 

 used, just as is the case in this experiment. The authors 

 call attention to the remarkable fact that in this case the 



