THE COMPOUND H^EMOLYSINS 



26l 



active in other similar cases. The proof of Ehrlich 

 and Sachs that an immune-body is not itself soluble in 

 (able to be bound by) ery throcytes, but only after combina- 

 tion with alexin, is henceforth untenable. 



I have made some experiments on the action of anti- 

 alexins and found that cases occur in which the influ- 

 ence of the antialexin has even a minimum value for a 

 medium concentration of the immune-body, and not a maxi- 

 mum, as in the theoretical example cited above. In one 

 case the erythrocytes were from sheep (i c.c. of a 

 5 per cent suspension) ; the immune-body (a), from a goat, 

 treated with erythrocytes from sheep the alexin (b) was 

 guinea-pig-serum ; and the antialexin (c) was from a goat 

 injected with serum from a rabbit. The quantity of alexin 

 was of such a magnitude that three-fourths of it would 

 be just sufficient to produce complete haemolysis. The 

 quantities are given in centimetres of the preparations used. 

 The experimental method was the same as that used by 



ACTION OF DIFFERENT QUANTITIES OF ANTIALEXIN 



