THE PRECttTTlNS AND THEIR ANTIBODIES 



2 7 



temperature (in mean 16 C.) during twenty to fifty min- 

 utes. Special experiments seemed to indicate that this time 

 of reaction has no notable influence if it only exceeds five 

 minutes, and after this time different quantities of the mix- 

 ture were added to 10 c.c. of milk and physiological salt- 

 solution added up to 12 c.c. The test-tubes containing 

 these mixtures were placed in a water-bath of constant 

 temperature and the coagulation examined after a given 

 time (two hours). The calculation for the experiment is 

 the same as for tetanolysin. One c.c. of the antirennet 

 was found equivalent to 1.48 times the fixed quantity of 

 rennet. The constant of equilibrium was found to be 

 K = 0.0 1 2. n is the quantity of antirennet used. 



NEUTRALISATION OF RENNET BY MEANS OF IMMUNE-SERUM FROM RABBIT 



The experiments are rather difficult and therefore a great 

 number of observations have been taken. The observed 

 values of the strength of the rennet in the mixture are 

 mean values of not less than eleven different measure- 

 ments. Thanks to this circumstance, it has been possible 

 to calculate the probable error of each value. This proba- 

 ble error is tabulated under A. As is seen from the 

 comparison of ^ calc . with g ^ the difference between the 

 calculated values and the observed ones are in eight cases 



