582 PRACTICAL ORGANIC AND BIO-CHEMISTRY 



Estimation of Creatine and Creatinine. 



The same procedure is carried out as above to obtain the picric acid filtrate ; 

 10 c.c. of it are placed in a flask which is covered with tin foil and heated in 

 an autoclave at 120 for 20 minutes ; the autoclave is opened after it has cooled. 



The solution is rinsed into a 25 c.c. measuring flask with saturated picric 

 acid solution, filled to the mark and 1*25 c.c. of sodium hydrate are added to 

 give the colour. 



2 or 3 standard creatinine solutions are required for the comparison on 

 account of the variations in the creatine content. 



i c.c. of 10 per cent, caustic soda is added to 20 c.c. of each of these. 



The comparison is made with the appropriate standard at 10 mm. 



The result is given by : 



reading of stand, x 125 x creatinine content in mgm. m ofcreatine + creatinine i n ro oc.c. 

 reading of unknown 



The amounts of the soluble nitrogenous extractives as determined by Folin 

 by his micro-methods in mgm. per 100 gm. of blood are as follows: 



In man higher values than the above for total nitrogen were found 

 in cases of syphilis and insanity. 



Man has more uric acid in his blood than any other mammal ; normal 

 blood contains 1-2 or 2*5 mgm : in cases of gout the amount is not greater 

 than 6 mgm. The output of uric acid in the urine according to Folin is a 

 question of kidney efficiency, since there is no greater output in the urine in 

 cases of gout, etc. The kidney action is selective and in cases of gout, etc., 

 the kidney is damaged with reference to uric acid and is unable to bring the 

 blood content down to the normal value. 



1 After 24 hours' fasting. 



