LIST OF REAGENTS 



613 



Add sodium acetate. 

 Alcoholic caustic soda. 



Ammonium sulphate 

 solution (sat.). 



Bangs reagent for 

 glucose estimation. 



Barfoetfs reagent. 



Benedict's qualita- 

 tive reagent for 

 glucose, etc. 



Benedicts quantita- 

 tive reagent for 

 gkicose, etc. 



Bertran&s reagents 

 for glucose estima- 

 tion. 



SPECIAL REAGENTS. 



ioo gm. sodium acetate ) are dissolved in water and 

 30 c.c. glacial acetic acid/ made up to i litre. 



20 gm. sodium, or \are dissolved in alcohol and made 

 20 gm. caustic soda/ up to i litre. 



780 gm. ammonium sulphate are dissolved in water and 

 made up to i litre. 



lare dissolved in the 

 ioo gm. potassium carbonate order iyen ^ 



66 gm. potassium chloride . about 6 c 



1 60 gm. potassium bicarbonate wa t e r at o 



ioo c.c. of 4*4 per cent, copper sulphate (or 4*4 gm.) 



are added and the whole diluted to 1000 c.c. after 



the carbon dioxide is evolved. The solution is 



only gently shaken so as to prevent entry of air. 



After 24 hours 300 c.c. are diluted to 1000 c.c. 



with sat. potassium chloride solution, shaken gently 



and used after 24 hours. 50 c.c. = 10 mg. glucose. 



66 gm. cupric acetate ) are dissolved in water and 

 10 c.c. glacial acetic acid/ made up to i litre. 



and dj . 



1 7 '3 g m - CuSO 4 . 5H 2 O are dissolved in ioo c.c. water 

 and diluted to 150 c.c. This solution is added with 

 constant stirring to the citrate-carbonate solution 

 contained in a beaker. The mixture is immediately 

 ready for use. 



are dissolved with the 

 aid of heat in enough 

 water to make about 

 800 c.c. volume, and 

 filtered. 



173 gm. sodium citratel are d j ssolv f d * 6o 

 , j water, filtered into a i litre 



ioo gm. anhydrous measuri 



sodium carbonate] 



("200 gm. Na 2 CO 3 . ioH 2 O 



I 



ioo gm. Na 2 CO 3 



200 gm. sodium citrate 



125 gm. KSCN 



Exactly 18 gm. of pure CuSO 4 . 5H 2 O are dissolved 



in ioo c.c. water and poured into the above solu- 



tion with constant stirring. 5 c.c. of 5 per cent. 



potassium ferrocyanide solution are added and the 



whole diluted to exactly i litre. 

 The addition of the trace of ferrocyanide prevents 



precipitation of red cuprous oxide. The solution 



apparently keeps indefinitely. 



'A. Copper sulphate 40 gm. ; water to 1000 c.c. 



B. Rochelle salt 200 gm 



NaOH 150 



C. Ferric sulphate 50 gm.j water to I000 c<c> 



H 2 SO 4 200 / 



water to 1000 c.c. 



KMnO^ 



5 gm. ; water to IOQO c.c, 



