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FRANK C. BECHT AND JAMES R GREER 



cipitation method which we used. They found bacterial precipitins in the cerebrospinal 

 fluid of immunized dogs and cats. 



The fluids used in these experiments were collected as described 

 at the beginning of this paper. The test tubes were the same as 

 those used in the hemolytic work. The bacteria were secured from 

 2o-24-hour slant agar cultures, made up with sterile 0.9 per cent 

 NaCl solution to a distinctly cloudy suspension, and then filtered 

 through filter paper to remove all of the clumps. A series of tubes 

 was arranged for each fluid, then after the proper amount of fluid had 

 been measured into each, the bacterial suspension was added. The 

 dilutions used were i : 10, i : 50, i : 100, i : 500, i : 2,000, i : 6,000. Our 

 lowest dilution was, perhaps, too high to detect the traces of agglutinins 

 reported by Pick and Kohler. The tubes were all incubated together 

 at 37 C. for two hours and then kept in the ice-box for from 12-20 

 hours before the final observations. 



In our study of agglutinins for the typhoid bacillus we used normal 

 cats and dogs, animals actively immunized by the repeated injection 

 of typhoid cultures, and animals rendered passively immune by the 

 withdrawal of large quantities of blood from the normal animal, and 

 the subsequent injection of an equal amount of warm, defibrinated 

 blood from an actively immunized animal of the same kind. 



A. Normal animals. We studied first the concentration of 

 agglutinins in the body fluids of normal cats and found agglutinins 

 for the typhoid bacillus active in a dilution from i : 10 in the serum 

 and the thoracic lymph; the neck lymph and the pericardial fluid 

 usually contain them in the same concentration but the cerebrospinal 

 fluid and the aqueous humor do not contain them in such dilution. 



DOGS. A study of the concentration of the typhoid agglutinins 

 in normal dogs gave the results shown in the following table : 



TABLE 6. 



THE COMPARATIVE AGGLUTINATING POWER ON THE TYPHOID BACILLUS OF THE BODY FLUIDS OF A 



NORMAL DOG. 



