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FRANK C. BECHT AND JAMES R. GREER 



was negative in all dilutions used in four of five cases, and agglutina- 

 tion in the fifth case at i : 10 was questioned. The aqueous humor 

 gave negative results in three of five cases, and the agglutination was 

 questioned in the remaining two at a dilution of i : 10. 



The development of agglutinins for the typhoid bacillus in dogs 

 runs a course which is strictly comparable to that in the cats under 

 similar circumstances. 



TABLE 8. 

 THE COMPARATIVE AGGLUTINATING POWER FOR THE TYPHOID BACILLUS OF THE BODY FLUIDS OF AN 



IMMUNE DOG. 

 (Immunized by repeated subcutaneous injections.) 



From Table 8 it is evident that the concentration of the agglutinins 

 in the body fluids of immune dogs runs a course parallel to that in the 

 immune cats. The concentration in the neck lymph and the peri- 

 cardial fluid is considerably lower than that in the thoracic lymph 

 and serum, and of the two the concentration in the neck lymph is the 

 higher. In our seven experiments on typhoid immune dogs, thoracic 

 lymph and serum showed the same concentration of bacterial agglu- 

 tinins in four cases; in the remaining three cases the concentration is 

 greater in the serum. The pericardial fluid contained agglutinins 

 in six of seven cases. The highest dilution at which agglutination 

 occurred was i : 100 (three cases) . In no case were agglutinins 

 found in the cerebrospinal fluid in the dilutions used. Traces of 

 agglutinins were observed in three of six cases in the aqueous humor 

 in a dilution of i : 10. 



We considered it of interest to determine whether immunity to 

 rabbit blood affected in any way the agglutinins for the typhoid 

 bacillus in the body fluids of a dog. With this in mind we tested the 

 usual six fluids of one of our immune dogs. An increased agglutinat- 

 ing power could be expected, if, in terms of Ehrlich's hypothesis, 

 there were any cell receptors common both to blood cells and the 

 typhoid bacilli. 



