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FRANK C. BECHT AND JAMES R. GREER 



the intestinal mucosa, and later the capillary or lymphatic wall, 

 depending upon whether the path of absorption was the blood or 

 the lymph. 



It was hoped that a careful study of the passage of these antibodies 

 from the blood into the various body fluids in a passively immune 

 animal would throw some light upon the problem of lymph formation. 

 We have been disappointed thus far, for our results have not been 

 decisive enough to warrant any general conclusions. 



Passive immunity was established by the withdrawal under light 

 ether anesthesia in most cases of a large amount of blood from a 

 normal animal, and the subsequent injection of an equal amount of 

 warm, defibrinated blood from an actively immune animal of the same 

 kind. 



CATS. To show the concentration of agglutinins in the body fluids 

 of a passively immune cat we give Table 10 as typical of our results. 

 It will also show the concentration of agglutinins of the actively 

 immune cat from which the blood was taken. 



TABLE io. 

 THE COMPARATIVE AGGLUTINATING POWER ON THE TYPHOID BACILLUS OF THE BODY FLUIDS OF CATS 



ACTIVELY AND PASSIVELY IMMUNE TO THE TYPHOID BACILLUS. 



(Cat n, immunized June 29 by the injection subcutaneously of six live 24-hour slant agar cultures 

 of the typhoid bacillus, operated July 9. Cat 12, passively immunized by the withdrawal on July 9 of 

 100 c.c. of blood and the injection of 100 c.c. of blood from Cat n. Operated July io.) 



* Normal serum before injection of immune serum 1 1 . 



From Table io it is evident that it is possible to increase the agglu- 

 tinins not only in the serum but in both of the lymphs by a process of 

 passive immunization. The relative concentration is maintained 

 in the fluids which we find in the actively immunized animals of the 

 same grade of immunity. In no case in the passively immunized 

 animal did the pericardial fluid show any increase over the normal 

 animal. The cerebrospinal fluid and the aqueous humor^ showed no 



