148 FRANK C. BECHT AND JAMES R. GREER 



which was tinged with blood were in good condition and were tested 

 in the usual manner. The results are shown in Table 1 1 . 



TABLE ii. 



THE RATE OF PASSAGE OF ANTIBODIES FROM THE BLOOD INTO THE BODY FLUIDS IN PASSIVELY 



IMMUNE ANIMALS. 



Highest Dilu- 



Fluid tion Showing 



Agglutination 



Normal serum 



Immune serum injected into passively immune dog. . 



Equal parts of normal and immune sera 



Serum 25 min. after transfusion 



" i hr. 25 min. after transfusion 



" 2 " " " " " 



14 3 " " " " " 



" 4 " " " " " 



Neck lymph 3 hr. " " 



4 " 30 min. after transfusion. . 



: 100 

 : 2,000 

 : 2,000 



: 5 

 .500 

 : 500 

 = 500 



'5 



Thoracic lymph 4 hr. 30 min. after transfusion i : 100 



Pericardial fluid 4 " 30 " " o 



Cerebrospinal fluid o 



Aqueous humor o 



It may be noted that the results of this experiment are practically 

 identical with those secured from our 24-hour experiments on the 

 passively immunized dogs. The concentration of the agglutinins of 

 the serum remain practically the same during the 4^ hours of this 

 experiment. The neck lymph had the same concentration of agglu- 

 tinins three hours after the transfusion that it had at the close of the 

 experiment. The thoracic lymph in this case showed considerably 

 higher than the neck lymph, part of which may have been due to the 

 serum in the lymph, which came either through the increased permea- 

 bility of the capillary walls due to the action of the anesthetic or to 

 trauma, or to the natural anastomoses between the lymphatics and 

 the blood vessels of the splanchnic area. 



The above experiment seems to indicate that the passage of such 

 substances as bacterial agglutinins from the blood to the lymphs is 

 a relatively rapid process, for the concentration of these bodies was 

 the same in the body fluids in 4^ hours as in 24 hours after passive 

 immunization. 



From the results obtained the following conclusions seem justified: 



i. Agglutinins for the typhoid bacillus are found in the serum and 

 thoracic lymph of normal cats in approximately equal amounts. 



