W*a 4 



i: 



CONCENTRATION OF ANTIBODIES 149 



They may or may not be present in the neck lymph and the pericardial 

 fluid. They are not found in the cerebrospinal fluid nor the aqueous 

 humor. 



2. Agglutinins for the typhoid bacillus are found in the serum, 

 neck lymph, and the thoracic lymph in normal dogs in relatively 

 higher concentrations than in the same fluids in normal cats. They 

 are most concentrated in the serum, are in nearly equal concentration 

 in the two lymphs. They may or may not be found in the pericardial 

 fluid. They are found neither in the cerebrospinal fluid nor the 

 aqueous humor. 



3. Agglutinins for typhoid bacilli are found in actively immune 

 cats in the serum, thoracic lymph, neck lymph, and pericardial 

 fluid in decreasing concentration in the order mentioned. If found 

 in the cerebrospinal fluid and aqueous humor there are only traces. 



4. Agglutinins for typhoid bacilli are found in actively immune 

 dogs in the serum, thoracic lymph, neck lymph, and pericardial 

 fluid, usually in decreasing concentration in the order named. Serum 

 and thoracic lymph may show an equal concentration. Cerebrospinal 

 fluid shows no agglutinins in a dilution of i : 10. Aqueous humor 

 may or may not show agglutinins in a dilution of i : 10. 



5. Immunization of a dog to rabbit blood does not increase the 

 concentration of agglutinins for typhoid bacilli in the body fluids 

 above the normal. 



6. In the passively immunized animal the agglutinins for typhoid 

 bacilli pass readily from the blood stream into the lymphs, usually in 

 greater concentration into the thoracic lymph than into the neck 

 lymph, but the concentration may be equal in the two, showing the 

 permeability of the two systems to be the same. They do not pass 

 into the pericardial fluid, cerebrospinal fluid, nor the aqueous humor. 



7. The passage of the agglutinins from the serum to the lymphs 

 in the passively immunized animal is a relatively rapid process, and 

 no difference is shown between the concentration at the end of 4^ 

 hours and 24 hours. 



OPSONINS. 



We made a quantitative study of the bacterial opsonins and the 

 hemopsonins in the body fluids of normal and immunized dogs. 



