FRANK C. BECHT AND JAMES R. GREER 



made, fixt, and stained in the ordinary way. Labels were then attached to the slides 

 and one of us numbered the slides in a haphazard manner and kept a record of the 

 numbering used. The slides were all mixt together and then counted by the other. 

 In this way the subjective factor would not enter at all. The results were highly 

 satisfactory, for by counting 500 leukocytes and estimating the percentage from that 

 number, the corresponding slides agreed almost exactly. In one case there was a 

 difference amounting to over 2 per cent. 



NORMAL DOGS. The first point was to determine the concentra- 

 tion of the hemopsonins in the body fluids of normal animals. As 

 a typical experiment we cite Table 14. 



TABLE 14. 

 COMPARATIVE HEMOPSONIC POWER FOR RAT CORPUSCLES OF THE BODY FLUIDS OF A NORMAL DOG. 



Control =0. 



This table shows that the concentration of hemopsonins in the 

 various body fluids runs practically parallel with that of the other 

 antibodies. It is highest in the serum. The two lymphs are lower 

 but practically equal. There are also traces in the other fluids in this 

 case. In another normal animal the same conditions were found. 



ACTIVELY IMMUNIZED DOGS. In establishing immunity for the 

 work on hemopsonins we followed a uniform technic. Each animal 

 received intravenously 0.5 c.c. of a 5 per cent solution of washed rat 

 corpuscles per kilo of body weight. The animal was operated the 

 loth day after the injection. We cite the experiment in Table 15 

 as typical. 



TABLE 15. 

 COMPARATIVE HEMOPSONIC POWER FOR RAT CORPUSCLES OF THE BODY FLUIDS OF AN IMMUNE DOG. 



Table 15 shows that a considerable degree of immunity was 

 reached, the immune serum producing a much higher degree of 



