CONCENTRATION OF ANTIBODIES 



phagocytosis than the normal. The concentration was highest in 

 the serum; the two lymphs are practically parallel with a slight balance 

 in favor of the neck lymph, because it not only produces a higher 

 percentage of phagocytosis in the lowest dilution, but also causes 

 phagocytosis in a much higher dilution. The pericardial fluid shows 

 a somewhat lower concentration than the lymphs; the cerebrospinal 

 fluid and the aqueous humor contain the opsonin in lower concentra- 

 tion than the pericardial fluid. 



In six experiments with immune dogs the concentration of hemop- 

 sonins ran the course shown in this experiment. We find some 

 variations in the cerebrospinal fluid and the aqueous humor. In 

 three out of five cases where the cerebrospinal fluid and aqueous 

 humor were tested the concentration of the hemopsonins was higher 

 in the former than in the latter. The fourth case is the one cited in 

 Table 15, and in the fifth case neither showed hemopsonins. In 

 one of six cases the hemopsonins of the pericardial fluid and aqueous 

 humor were practically equal in concentration. 



PASSIVELY IMMUNE DOGS. Passive immunity was produced in 

 the same way described under bacterial agglutinins. We cite here 

 as an example the results obtained in the case of a dog immunized 

 by injecting intravenously 300 c.c. of the blood of an immune dog 

 after first removing 260 c.c. by bleeding. 



TABLE 16. 



COMPARATIVE HEMOPSONIC POWER FOR RAT CORPUSCLES OF THE BODY FLUIDS OF A DOG PASSIVELY 



IMMUNIZED TO RAT BLOOD. 



The results in our experiment in passive immunity show first, an 

 increase in the concentration of the hemopsonins in the serum, and 

 second, a marked increase in the concentration of the antibodies in 

 the thoracic lymph, bringing it up to a concentration equal to that of 

 the serum, while the concentration of the hemopsonins in the neck 



