156 FRANK C. BECHT AND JAMES R GREER 



lymph remains low and from a comparison with other animals we 

 would say that the injection of the highly immune serum had had no 

 effect upon the concentration of the hemopsonins in the neck lymph. 

 The same is true of the pericardial fluid, the cerebrospinal fluid, and 

 the aqueous humor, where the concentration is no higher than in the 

 normal dog. It must be remembered that this dog showed a relatively 

 high hemopsonic power before immunization by injection of the 

 immune blood. We have other experiments which show the same 

 phenomenon. 



From these experiments we draw the following conclusions : 



1. Hemopsonins are found in the body fluids of normal dogs. 

 The concentration is highest in the serum, lower in the thoracic and 

 neck lymph, which run almost parallel, and are found in the other 

 body fluids only in traces. 



2. By a process of immunization the hemopsonins of the body 

 fluids can all be increased. The order of descending concentration 

 is the serum, neck lymph, thoracic lymph, pericardial fluid, aqueous 

 humor, and the cerebrospinal fluid. Sometimes the arrangement in 

 the scale of the last two is reversed. 



3. Hemopsonins can pass from the blood into the thoracic lymph, 

 but apparently they do not pass into the lymph of the head region. 

 The concentration of the hemopsonins in the pericardial fluid, cere- 

 brospinal fluid, and aqueous humor is not increased in 24 hours over 

 the normal by passive immunization as in our experiments. 



GENERAL CONCLUSIONS. 



In the normal animal the concentration of hemolysins, hemag- 

 glutinins, bacterial agglutinins, bacterial opsonins, and hemopsonins 

 decrease in the body fluids in the following order: serum, thoracic 

 lymph, neck lymph. Traces of hemagglutinins, bacterial agglutinins, 

 and opsonins are found in the pericardial fluid. Traces of opsonins 

 are found in the cerebrospinal fluid and aqueous humor. No pre- 

 cipitins for rabbit serum were found in any of the body fluids. 



Immunization increases the concentration of the antibodies named 

 above in the fluids in which they are found in the normal animal. 

 Hemolysins are sometimes found in the pericardial fluid, and in a 

 few cases traces of bacterial agglutinins in the cerebrospinal fluid and 



