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FRANK C. BECHT AND JAMES R. GREER 



repeated, small subcutaneous injections. A comparison of the 

 results secured by using the fluids directly from the animal, so far as 

 the lysins are concerned, is not conclusive, for, as will be seen, com- 

 plement is not increased, at least not in proportion to the ambocep- 

 tors, if at all. This fact necessitates the use of sufficient complement 

 to supply all the amboceptors present to demonstrate the true state 

 of affairs. So far as the agglutinins are concerned, apparently a 

 single large dose of the serum or blood may develop them more 

 markedly than the other methods tried. The repeated, small intra- 

 peritoneal injections yielded the most uniform results. 



As has been noted by numerous investigators, the increase in 

 complement does not keep pace with the increase in amboceptors. 

 The apparent increase in the hemolysins and in the agglutinins for 

 rabbit corpuscles is shown in Table 2. 



TABLE 2. 



LYTIC AND AGGLUTINATING ACTION OF THE BODY FLUIDS OF A DOG IMMUNIZED WITH RABBIT BLOOD. 



(November 25, December 15, 10 c.c. rabbit blood intraperitoneally; December 15, 



15 c.c. Fluids collected December 23.) 



From Table 2 it can be seen by comparison with Table i that the 

 repeated injections of rabbit blood does not appear to increase to any 

 very marked extent the hemolytic power of the body fluids over that 

 of a normal animal. 



There is, however, a marked increase in the power of the body 

 fluids to agglutinate rabbit corpuscles in those fluids which had the 

 power to agglutinate them previous to the injection; there is no 

 development, except in a few cases, of agglutinins in the cerebrospinal 

 fluid and the aqueous humor. Altho Table 2 does not show this 

 point, a careful comparison of this table with the succeeding ones 



