< x r 



CONCENTRATION or ANTIBO^ES^ 133 



humor of normal animals contain no lysins or agglutinins for rat or 

 rabbit corpuscles. In our 10 experiments on normal animals there 

 were no traces of hemolysis or agglutination in a single case where 

 admixture of blood was eliminated. Our results with cerebrospinal 

 fluid confirm those of Massaglia, who could find no lysins in that 

 fluid, and are contrary to those of Bard, who claims to have demon- 

 strated them there. 



Conclusions. i. In the normal dog hemolysins for rabbit cor- 

 puscles are found in the serum, neck lymph, and thoracic lymph, 

 but are absent from the pericardial fluid, cerebrospinal fluid, and 

 aqueous humor. They are most concentrated in the serum, less 

 concentrated in the thoracic lymph, and are found only in traces in 

 the neck lymph. 



2. Agglutinins are found in the serum, neck lymph, and thoracic 

 lymph of normal dogs. They may or may not be present in the 

 pericardial fluid, and are always absent from cerebrospinal fluid 

 and aqueous humor. In most cases the concentration descends in 

 the following order: serum, thoracic lymph, neck lymph, pericardial 

 fluid; altho in some cases, the order is thoracic lymph, serum, neck 

 lymph, pericardial fluid. 



3. Serum and thoracic lymph show a weaker hemolysis and 

 agglutination toward rat than toward rabbit corpuscles. Neck 

 lymph lakes and agglutinates rabbit but not rat corpuscles. Peri- 

 cardial fluid agglutinates rabbit but not rat corpuscles. Cerebro- 

 spinal fluid and aqueous humor neither lake nor agglutinate rat or 

 rabbit corpuscles. 



B. Immunized animals. Various methods of producing active 

 immunity were employed with good success. It is of interest to 

 ascertain what methods of immunizing yield the best results. We 

 employed the following: (i) Immunization of dogs with rabbit blood : 

 (a) intraperitoneally by a single large injection of from 80 to 150 c.c. 

 of blood, (b) intraperitoneally by repeated small injections, (c) sub- 

 cutaneously by repeated small injections. (2) Immunization of dogs 

 with horse serum:* (a) by a single large intraperitoneal injection of 

 100-150 c.c., (b) by repeated, small intraperitoneal injections, (c) by 



* This serum was secured aseptically, November, 1908, by drawing the blood from the carotid of 

 a horse into jars. It was allowed to coagulate and stand in the ice-box until the serum came out. The 

 serum was then sealed into bulbs and kept in the ice-box until used. 



