CONCENTRATION OF ANTIBODIES 



This difference in the hemolytic power of serum or other body fluid 

 against the corpuscles of different species of animals has been 

 explained by Ehrlich and his coworkers on the basis of a multiplicity 

 of amboceptors and complements, some of which are specific, some 



TABLE i. 



COMPARATIVE HEMOLYTIC AND AGGLUTINATING POWER OF THE BODY FLUIDS OF A NORMAL DOG ON 

 RABBIT AND RAT CORPUSCLES. 



*It will be noted that in this table several tubes are marked "sp." By that symbol is meant 

 hemolysis not due to the ordinary hemolysins. The appearance of the partially laked corpuscles is entirely 

 different from that in the ordinary hemolytic test. The hemoglobin can be seen diffusing from the sedi- 

 mented corpuscles, while the supernatant fluid remains perfectly clear. The hemoglobin has the peculiar 

 reddish purple tint of reduced hemoglobin, instead of the clear red of oxyhemoglobin. Furthermore, 

 laking may appear anywhere in the series, frequently, where no hemolysis is to be expected, and is met 

 more often in fluids like the cerebrospinal, or aqueous humor, which are normally not hemolytic, than in 

 the other fluids. Rat corpuscles seem more susceptible to this form of hemolysis than rabbit corpuscles. 

 Complement seems to inhibit this form of hemolysis. 



non-specific. In most cases the thoracic lymph of normal dogs is 

 hemolytic for rat corpuscles, altho in the experiment cited above, such 

 was not the case. 



As may be seen from the table above, the concentration of agglu- 

 tinins may be higher in the thoracic lymph than in the serum. Such, 

 however, is not the usual finding. In 10 experiments on normal 

 dogs we found in seven the concentration of agglutinins highest in 

 the serum; in two it was highest in the thoracic lymph; and in one 



