BECHT AND JAMES R. GREER 



rabbit corpuscles in the body fluids to form a descending series: serum, thoracic lymph, 

 neck lymph, pericardial fluid, aqueous humor. No lysins were found in the cere- 

 brospinal fluid. Straus and Wolf4 studied the hemolytic power against rabbit cor- 

 puscles of the cerebrospinal fluid, edema fluid, pleural and pericardial transudates, 

 and blister fluid, and attempted to correlate the hemolytic strength with the protein 

 content. Marshall and Morgenroth 2 s found anti-complement and anti-amboceptor 

 in a pathological exudate an ascites fluid. Hedinger j s studied the hemolytic power 

 of non-inflammatory exudates like those arising from cirrhosis of the liver and heart 

 failure, and found that they were not so hemolytic as the serum. The inflammatory 

 exudates arising from cases of tuberculosis and carcinoma were not so strongly hemolytic 

 as non-inflammatory exudates. He failed to find hemolysins in the fluid from an 

 ovarian cyst, or in the cerebrospinal fluid in two cases of tuberculosis. Marshall 2 ^ 

 found that pleural and ascites fluids were more strongly hemolytic than the serum 

 from an infant. But no conclusions can be drawn from this comparison in regard to the 

 comparative hemolytic power of serum and other body fluids in the same individual. 

 He found a multiplicity of amboceptors and complements in the fluids that he studied. 

 Grollo 4 could find no amboceptors for rabbit corpuscles in transudates, but found 

 them in exudates, altho in the latter complement is often lacking. He suggests this 

 method as a means of diagnosis between transudates and exudates. Liidke 22 con- 

 firmed the findings of Marshall in regard to the hemolytic strength of transudates and 

 exudates. Granstronvs found wide variations in the hemolytic content of transu- 

 dates and exudates, and could establish no characteristics essential for either. The 

 hemolysins did not run parallel with those of the blood. Isolysins are found less 

 frequently in transudates and exudates than in the blood. Hemolysins were not 

 found in the cerebrospinal fluid. Isolysins and heterolysins were found independent 

 of the albumen content, number of the leukocytes, and the osmotic pressure of the 

 fluids tested. Tedeschi4; found precipitins in both transudates and exudates, less 

 frequently in the latter than in the former. Mioni3 found amboceptor but no com- 

 plement for guinea-pig corpuscles in the pericardial fluid of the ox. Bard 2 claims to 

 have found hemolysins in the cerebrospinal fluid of patients, and found that they were 

 increased during various diseases. Massaglia 26 could not confirm the work of Bard. 

 His results in both healthy and diseased individuals were negative. The presence of 

 antibodies for syphilitic material in the cerebrospinal fluid has been shown by various 

 investigators, among them Morgenroth and Stertz,3i and Wassermann and Plaut.4 2 

 Gatti 2 could demonstrate no hemolysins in the aqueous humor of the ox. Levaditi 2 

 showed that there is normally no opsonin in the aqueous humor; but if the fluid of the 

 anterior chamber of the eye of an immune animal is withdrawn, the newly formed 

 aqueous humor will contain opsonin. Bohme 6 investigated the opsonin content of 

 pleural, peritoneal, and abscess fluids He found that usually in such cases the opsonin 

 content of the fluid was reduced for the infecting organism, but remained unchanged 

 for other bacteria. He could find no opsonin in normal cerebrospinal fluid, but found 

 them there after an inflammation had been set up in the dura. He could not develop 

 opsonins in the cerebrospinal fluid by repeated puncture as Levaditi had done by 

 drawing off the aqueous humor. He believes that there is a relation between the 

 protein content and the opsonin action of a fluid. 



Methods. The plan of study adopted was to determine first the concentration 

 of the antibodies in the body fluids of normal cats and dogs; then the concentration in 

 actively immunized animals; and, finally, to study the passage of the antibodies from 



