72 AXILIN COLOURS GIVING INDIRECT NUCLEAR STAINS. 



The mordanting action of permanganate of potassium on 

 anilin stains is so energetic that if it have been overmuch 

 prolonged before staining with safranin, or, still more, with 

 rubin, it becomes almost impossible to wash out the sections 

 properly; it may be necessary to leave them for nearly a 

 month in clove oil. 



Heneguy's preparations are certainly most successful, and 

 I most highly recommend the method for all preparations in 

 which it is desired to have the achromatic cell-structures 

 stained at the same time as the chromatic elements. And I 

 think it may be recommended to all workers who feel the 

 want of a means of slowing the sometimes inconveniently 

 rapid decoloration of sections treated by the F lemming 

 method. 



107. Other Nuclear Stains by the Indirect Method. The fore- 

 going paragraphs nearly exhaust the list of colours giving good nuclear 

 stains by the indirect process. FLEMMING (Arch. f. mik. Anat., xix, 1881, 

 pp. 317 and 742) mentions the following : 



MAGDALA RED (NAPHTHALIN RED, ROSE DE NAPHTHALINE). Nearly if 

 not quite as good a stain as any of the foregoing, and superior to all except 

 safranin in respect of permanency. This and the following should, as far 

 as is yet made out, be used in alcoholic solution diluted with about one half 

 of water, and be washed out with pure alcohol, followed by clove oil. 



Mauvein and Rouge Fluorescent are good stains, but colour some 

 nuclei more deeply than others in the same preparation. 



Solid Green (perhaps the same as the anilin green discussed above) is 

 very elective for nucleoli. 



Fuchsin (meaning the basic fuchsins, a series of Rosanilin salts having 

 very similar reactions and found in commerce under the names of FUCHSIN, 

 ANILIN RED, RUBIN, ROSEIN, MAGENTA, SOLFERINO, COEALLIN). A good 

 but somewhat weak stain, by the alcohol method. Good results are obtained 

 by substitution in the following manner (GBASEE, Deutsche Zeit. f. Chi- 

 rurgie, xxvii, 1888, pp. 538584 ; Zeit. f. wiss. Mile., v, 3, 1888, p. 378). 

 You either employ the colour as directed for methyl violet (post, 111), or 

 you stain for twelve to twenty-four, hours in a dilute aqueous solution, wash 

 out for a short time in alcohol, stain for a few minutes in aqueous solution 

 of methylene blue, and dehydrate with alcohol. A double stain. Chromatin 

 and nucleoli red, all the rest blue. 



ZIEHL'S CARBOLIC FUCHSIN has been recommended as supe- 

 rior to safranin by SCHENK (Ueber Conservirung von Kerntheil- 

 ungsfiynren, Bonn, 1890). I do not know where the original 

 formula was published, and take this from Zeit.f. wiss. Mik. 

 (vii, 1, 1890, p. 39). 



