158 COMBINATION STAINS. 



stain. This one, communicated to Fol by Renaut, is the 

 latest, and I suppress the others. 

 Take- 

 Concentrated aqueous solution of potassic eosin 



(eosine a la potasse) . . . . .30 c.c. 



Saturated solution of haematoxylin in alcohol 

 (ought to have been kept some time and to 

 have precipitated) . . . . . .40 c.c. 



Saturated solution of potash alum in glycerin 



(of a density of about 1'26) ... 130 c.c. 

 Mix, and let the mixture stand five or six weeks in a vessel 

 covered with a sheet of paper pierced with holes until the 

 alcohol is evaporated, then filter. 



For staining, the solution may be used as it is or diluted. 

 Staining goes on very slowly, and at first the colour is not 

 held by the tissues, but disappears on washing. After some 

 days or weeks, however, it becomes localised and fixed in the 

 tissues. You may then mount in balsam, taking care to 

 employ alcohol charged with a sufficient quantity of eosin. 

 But it is frequently preferable to proceed by mounting the 

 objects in the staining fluid diluted with one to two volumes 

 of glycerin. After a few weeks this mounting medium will 

 have become perfectly colourless through the absorption of 

 the colour by the tissues. 



The stain has a specific action on the cells of salivary and 

 gastric glands. Mucus-cells become pale blue ; salivary 

 ferment-cells (crescent-cells of Gianuzzi) intense rose. 



There is no doubt as to this being a very fine and useful 

 stain. See also Comptes Rendus, 1879, p. 1039 (Ire ser.), 

 and Arch, de PhysioL, 1881, p. 640. 



253. Haematoxylin and Benzopurpurin (ZSCHOKKE). For this 

 and the combination with Delta Purpurin, see ante, 133. 



254. Haematoxylin and Rubin and Orange. Mr. A. PRINGLE 

 informs me that this is an admirable combination. You 

 should stain in EHELICH'S hsematoxylin, wash in distilled or 

 even acidified water, blue the stain with tap water, or water 

 with a trace of alkali added to it, then stain in the rubin and 

 orange. 



255. Heematoxylin and Iodine Green (STIRLING, Journ. of Anat. 

 and PhysioL, xv, 1881, p. 353). Heematoxylin and Nitrate of Kosa- 



