192 IMBKDDING METHODS. 



gelatin, 40 grms. ; saturated solution of arsenious acid, 200 

 c.c. ; glycerin, 120 c.c. Clarify with white of egg. The 

 mass may be kept for years in a well-stoppered bottle. The 

 objects to be prepared for imbedding by a bath of one third 

 glycerin. 



296. BRUNOTTI'S Cold Gelatin Mass (Journ. de Botan., vi, 

 1892, p. 194; Journ. Roy. Mic. Soc., 1892, p. 706). Twenty 

 grms. gelatin dissolved with heat in 200 c.c. distilled water, 

 and 30 to 40 c.c. of glacial acetic acid with 1 grm. corrosive 

 sublimate added after filtering. At the temperature of 

 15 C. the mass has the consistence of a thick syrup. Objects 

 are prepared by soaking in some of the mass diluted with two 

 to three vols. of water, then imbedded in the undiluted mass. 

 The mass is then hardened in spirit or bichromate of 

 potash, picric acid, or the like. No heat at all is required in 

 this process. 



