EIPAET AND PETIT's FLUID. 239 



4TH FLUID. " Marine animals require a stronger fluid of 

 this kind, made by adding about 2 ounces more salt to 

 the last." 



383. Owen's Fluid (quoted from VOGT et YUNG, Traite 

 d'Anat. comp. pratique, p. 19). 



Corrosive sublimate . . . 0*014 grm. 

 Alum ...... 79 grms. 



Salt 137 



Water 1680 



Said to be very useful for the preservation of soft-bodied 

 animals. 



384. Gilson's Fluid (CARNOY'S Biologie cellulaire, p. 94). 

 Alcohol of 60 per cent. . . .60 c.c. 



Water 30 



Glycerin . . . . . 30 

 Acetic acid (15 parts of the glacial 



to 85 of water) . . . 2 



Bichloride . . . . O'lS grm. 



A really excellent medium for the study of fine cellular 

 detail with well-fixed objects. 



385. Gage's Albumen Fluid (Zeit. f. wiss. Mik., 1886, p. 

 -223). 



White of egg .... 15 c.c. 



Water 200 



Corrosive sublimate . . . 0'5 grm. 



Salt 4 grms. 



Mix, agitate, filter, and preserve in a cool place. Recom- 

 mended for the study of red blood-corpuscles and ciliated cells. 



Other Fluids. 



386. Chloride and Acetate of Copper (RIPART et PETIT'S fluid, 

 Brebissonia, 1880, p. 92 ; CAKNOY'S Biol. cell., p. 95). 



Camphor water (not saturated) . 75 grms. 

 Distilled water .... 75 

 Crystallised acetic acid ... 1 grm. 

 Acetate of copper .... 0*30 

 Chloride of copper . . . 0*30 



This is certainly a most valuable medium for work with 

 delicate fresh tissues. It may be used in combination with 



