LANDOIS'S SOLUTION. 285 



four to forty-eight hours, stain with fuchsin, eosin, or picro- 

 carmine. 



If a crystalline lens be macerated as above its fibres become 

 beaded or moniliform. 



531. Saliva, Artificial (for embryology of nerve and muscle) 

 (CALBERLA'S formulae, Arch. f. mik. Anat., xvi, 1879, p. 471, 

 e l se q f ). After having made trial of various different mace- 

 rating agents, with the object of obtaining isolation of the 

 developing muscle and nerve of embryos of Amphibia and 

 Ophidia, Calberla found that the best results were obtained 

 by means of Czerny's mixture of saliva and solutio Mulleri. 

 This led him to imagine an artificial saliva, which on trial 

 gave results as good as those obtained by natural saliva, or 

 even better. 



Second formula (the first formula is suppressed, as being 

 more complicated, and not giving better results) : 



Potassium chloride .... 0*4 

 Sodium chloride ..... 0'3 

 Phosphate of soda .... 0*2 

 Calcium chloride ..... 0*2 



M 



This is dissolved in 100 parts of water, saturated with 

 carbonic acid, and the solution combined with water and 

 solutio Mulleri, one volume of the solution being combined 

 with half a volume of Miiller's solution and a volume of 

 water. 



In either case the Miiller's solution may be replaced by a 

 2J per cent, solution of chromate of ammonia. The best 

 results were obtained when the solutions were saturated with 

 the CO 2 just before using. 



The tissues are isolated by teasing and shaking, and speci- 

 mens mounted in concentrated acetate of potash. 



532. LANDOIS'S Solution (Arch.f. mik. Anat., 1885, p. 445). 

 Saturated sol. of neutral chromate of ammonia 5 parts. 

 Saturated sol. of phosphate of potash . 5 



Saturated sol. of sulphate of soda . . 5 



Distilled water 100 



To be used in the same way as chromic acid : Small pieces 

 of tissue are macerated for one to three, or even four or five 



