286 MACERATION AND DIGESTION. 



days, in the liquid, then brought for twenty -four hours into 

 ammonia carmine diluted with one volume of the macerating 

 liquid. 



GIERKE particularly recommends this liquid for all sorts of 

 macerations, but especially for the central nervous system, for 

 which he finds it superior to all other agents. It is also re- 

 commended for the same purpose by NANSEN (v. Zeit. f. wiss. 

 Mik., v, 2, 1888, p. 242). 



533. Permanganate of Potash, Has an action similar to that 

 of osmic acid, but more energetic. Is recommended, either 

 alone or combined with alum, as the best dissociating agent 

 for the fibres of the cornea (ROLLETT, Strieker's Handbuch, 

 p. 1108). 



534. Chromic Acid. Generally employed of a strength of 

 about 0'02 per cent. Specially useful for nerve tissues and 

 smooth muscle. Twenty-four hours' maceration will suffice 

 for nerve-tissue. About 10 c.c. of the solution should be 

 taken for a cube of 5 mm. of the tissue (Ranvier). 



535. Bichromate of Potash. 0*2 per cent. 



536. Muller's Solution. Same strength. 



537. Muller's Solution and Saliva (see above, 531). 



538. BROCK'S Medium (for nervous system of Mollusca, 

 Intern. Monatsch. /. Anat., i, 1884, p. 349). Equal parts of 

 10 per cent, solution of bichromate of potash and visceral 

 fluid of the animal. 



539. MOBIUS'S Media (quoted from Zeit. f. wiss. Mik., iii, 3, 

 1886, p. 402). 



1. One part of sea water with 4 to 6 parts of 0*5 per cent, 

 solution of bichromate of potash. 



2. 0*25 per cent, chromic acid, O'l per cent, osmic acid, 

 0*1 per cent, acetic acid, dissolved in sea water. For Lamelli- 

 'branc'hiata. Macerate for several days. 



540. GAGE/S Picric Alcohol (Proc. Amer. Soc. of Microscopists, 

 1890, p. 120; Zeitf. wiss. Mik., ix, 1, 1892, pp. 87, 88). 95 

 per cent, alcohol, 25Q parts; \\rater, 750; picric acid, 1.' 

 Recommended for most tissues, but especially for epithelia 

 and smooth and striated muscle. This is also much recom- 



