GEDOELST'S METHODS. 259 



distilled water, and the solution filtered. It filters readily. 

 Or the powder may be dissolved in glycerin. The tissues to 

 be digested may be kept for some hours in the liquid at a 

 temperature of 100 F. (37 C.). 



550. BRUCKE'S Digestion Fluid (from CARNOY'S Biologie cellu- 

 laire, p. 94). 



Grlycerinated extract of pig's stomach. . 1 vol. 

 0'2 per cent, solution of HC1 . . . 3 vols. 

 Thymol, a few crystals. 



551. BICKFALVI'S Digestion Fluid (Centralbl. f. d. med. Wiss., 

 1883, p. 833). One grm. of dried stomachal mucosa is mixed 

 with 20 c.c. of 0*5 per cent, hydrochloric acid, and put into an 

 incubator for three or four hours, then filtered. Macerate 

 the tissue in the solution for not more than half an hour to an 

 hour. 



552. KUSKOW'S Digestion Fluid (Arch. f. mik. Anat., xxx. 

 p. 32; cLZeit.f. wiss. Mik., iv, 3, 1887, p. 384). One part of 

 pepsin dissolved in 200 parts of 3 per cent, solution of oxalic 

 acid. The solution should be freshly prepared, and the 

 objects (sections of hardened Ligamentum Nuchae) remain in 

 it at the ordinary temperature for ten to forty minutes. 



553. SCHIEFFERDECKER'S Pancreatin Digestion Fluid (Zeit. f. 

 wiss. *Mik., iii, 4, 1886, p. 483). Solution of pancreatin in 

 water. Schiefferdecker employs the " Pankreatinum siccum" 

 prepared by Dr. Witte, Eostock. A saturated solution is 

 made in distilled water, cold, and filtered. Pieces of tissue 

 (epidermis) are macerated in it for three to four hours at 

 about body temperature. Nuclei are preserved, and the 

 forms of prickle-cells well shown. 



554. KUHNE'S Methods (see Unters. a. d. Phys. Inst. Univ. 

 Heidelberg, i, 2, 1877, p. 219). Trypsin is prepared by ex- 

 tracting ox-pancreas with alcohol and ether, and evaporating 

 to dryness. One part of the product is heated for three to 

 four hours at a temperature of 40 C., with 5 to 10 parts of 

 O'l per cent, solution of salicylic acid, and the solution forced 

 through linen and filtered cold. 



554 a. GEDOELST'S Methods (see La Cellule, iii, 1887, p. 117, 

 and v, 1889, p. 126; also Zeit. f. wiss. Mik., vii, 1, 1890, 

 p. 57). 



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