322 EMBRYOLOGIOAL METHODS. 



very dilute Kleinenberg's haernatoxylin. Imbed in paraffin, 

 giving a short paraffin bath (not more than ten to fifteen 

 minutes). 



For study of entire specimens in the early stages, Perenyi's 

 liquid, followed by alcohol, water, very dilute iodine solution, 

 and glycerin, gives the best results. 



631. Ascaris. See the chapter on " Cytological Methods." 



Echinodermata, Coelenterata, and Porifera. 



See the paragraphs treating of these groups in Chap. 

 XXXII. 



For the maturation and fecundation of the ova of the Echi- 

 nodermata, see also the chapter on " Cytological Methods." 



