SMOOTH MUSCLE ISOLATION OP FIBEES. 355 



greatly). The methods only succeed completely during fine 

 sunny weather. 



673. Corpuscles of Golgi (CATTANEO, Arch. ital. de Biol., x, 

 1888, p. 337). The method here recommended is the arsenic 

 acid method of Golgi that has been described above, 670, 

 sub voce " KUHNE " (4) . 



See also RUFPINI (Atti E. Ace. Lincei Roma, 1892, p. 442; 

 Zeit.f. wiss. Mik., ix, 2, 1892, p. 237), who recommends the 

 method of Fischer. 



674. Corpuscles of Golgi (CiACCio, Mem. R. Ace. Sci. Bologna 

 (4), t. x, 1890, p. 301 ; Zeit.f. wiss. Mik., vii, 4, 1891, p. 507). 



For Amphibia the usual gold methods are not satisfactory, 

 because the ground-substance of the tendon takes the stain 

 at the same time as the nerve- endings. Pieces of tendon 

 should be put into 0*1 per cent, hydrochloric acid or 0*2 per 

 cent, acetic acid until quite transparent. They should then 

 be put for five minutes into a mixture of 0*1 per cent, gold 

 chloride and O'l per cent, potassium chloride. After that 

 they are put back into the acetic acid, and remain there for 

 a day in the dark, and for two or three hours more in the 

 sunlight. When they have become somewhat violet they are 

 put for a day into 0*1 per cent, osmic acid, and finally mounted 

 in Price's glycerin acidulated with 0*5 per cent, of acetic or 

 formic acid. 



675. The Methylen-blue Method. I find no mention of the 

 application of this method to the study of the relations of 

 nerve and tendon, for which it would seem a priori to be pecu- 

 liarly suitable. 



Smooth Muscle. 



676. Smooth Muscle Isolation of Fibres (SCHWALBE, Arch.f. 

 mik. Anat., 1868, p. 394). Maceration in weak chromic acid 

 solution (0-02 per cent, proved a generally useful strength). 

 This is a better reagent than osmic acid, 1 per cent, acetic 

 acid (Moleschott), weak sulphuric acid, pyroligneous acid 

 (Meissner), 20 per cent, nitric acid (Reichert), 32 to 35 per 

 cent, potash solution (Moleschott), as it preserves better than 

 any of these the finer structure of the cells. 



GAGE'S methods. See Journ. Roy. Mic. Soc., 1887, p. 327; 

 and 529, 540, and 544, ante. 



