356 MUSCLE AND TENDON. 



MOBIUS, liquid for maceration of the muscle of Oardium (see 

 above, 539). 



BALLOWITZ, muscle of Cephalopoda, see Arch. f. mik. Anat., 

 xxxix, 1892, p. 291 ; Zeit.f. wiss. Mik., ix, 3, 1893, p. 344. 



677. Bladder of Frog, Innervation of (WOLFF, Arch. f. mik. 

 Anat., 1881, p. 362). A frog is killed and a solution of gold 

 chloride of 1 : 20,000 injected into the bladder through the 

 anus. (If the injection flows out on removal of the syringe, 

 tie the frog's thighs together.) Now open the frog, dissect 

 away the attachments of the bladder, ligature the intestine 

 above the bladder, and cut away the abdomen of the frog so 

 as to have in one piece bladder, rectum, and hind legs. (All 

 this time the bladder must be kept moist with weak gold 

 solution.) The bladder and the rest are now put into gold 

 solution of 1 : 2000 for four hours ; the bladder is then ex- 

 cised, slit open, and pinned (with hedgehog spines) on to a 

 cork (outside downwards). Place it under running water 

 until all the epithelium is washed away. Use a pencil if 

 necessary. Put for twenty-four hours into gold solution of 

 1 : 6000. Wash in pure water, and put away in the dark "for 

 some time" in acidulated water, and finally reduce in fresh 

 water in common daylight. The muscles should be pale blue- 

 red; medullated nerves dark blue-red; sympathetic nerves 

 and ganglia carmine-red. KANVIER (Traite, p. 854) recom- 

 mends one or the other of his two gold processes. The 

 bladder of frogs should be carefully distended by injection of 

 the lemon juice or gold chloride and formic acid through the 

 cloaca. 



The methylen-blue method would appear to be very much 

 indicated for this subject, but has not been tried so far as I 

 am aware. 



678. Musculus dilatator Pupillae (DOGIEL, Arch.f. mik. Anat., 

 1886, p. 403). An enucleated eye is divided into halves, and 

 the anterior one with the iris brought for some days into a 

 mixture of two parts one-third alcohol and one part 0'5 per 

 cent, acetic acid. The iris can then be isolated, and split 

 from the edge into an anterior and posterior plate, and these 

 stained according to the usual methods. 



679. Iris (KOGANEI, Arch.f. mik. Anat., 1885, p. 1). The 

 pigmented epithelium can be removed by brushing with a 



