TEST FOB SMOOTH MUSCLE. 357 



small brush after prolonged maceration in solution of Miiller. 

 The pigment may also be bleached by chlorine water, which, 

 however, should only be allowed to act for a few hours, until 

 the pigment has become of a light brown ; complete de- 

 coloration may be obtained by prolonging the reaction for 

 twenty-four hours, but then the tissues suffer. (See Journ. 

 Roy. Mic. Soc., 1886, p, 874.) 



See also CANFIELD, in Arch.f. mik. Anat., 1886, p. 121 ; and 

 DOSTOIEWSKY, ibid., p. 91 (sections stained with haema- 

 toxylin and eosin, or [LiST, Zeit.f. wiss. Mik. f iii, 4, 1886, p. 

 514] with Renaut's hsematoxylic glycerin). 



680. Stomach of Triton (see STILLING and PFITZNER, in Arch, 

 mik. Anat., 1886, p. 396). 



681. Test for Smooth Muscle (RETTERER, Comptes Rend. Soc. 

 Biol., iv, 1887, p. 645; Journ. Roy. Mic. Soc., 1888, p. 843). 

 If a specimen of tissue be fixed in a mixture of ten vokimes 

 of 90 per cent, alcohol and one volume of formic acid, well 

 washed, and stained for twenty-four to thirty-six hours with 

 alum- carmine, the cytoplasm of smooth muscle will be found 

 to be stained red, whilst connective-tissue cells remain un- 

 stained, and are swollen. 





