362 NERVES. 



687. Other Methods. WALDEYER, Strieker's Handb., p. 958 (decalci- 

 fication either in O'OOl per cent, palladium chloride containing 10 per cent, 

 of HC1, or in chromic acid of O25 to 1 per cent.). 



UBBAN PBITCHARD (Journ. Roy. Mic. Soc., 1876, p. 211). Decalcifica- 

 tion in 1 per cent, nitric acid. 



LAVDOWSKY (Arcli.f. mik. Anat., 1876, p. 497). Fresh tissues (from the 

 cochlea) are treated with 1 per cent, solution of silver nitrate, then washed 

 for ten minutes in water containing a few drops of 0'5 or 1 per cent, osinic 

 acid solution, and mounted in glycerin. 



MAX FLESCH (Arch.f. mik. Anat., 1878, p. 300). 



TAPANI (Arch. Ital. de Biol., vi, p. 207). 



POLITZER, 4( Die anatomische u. histologische Zergliederung 

 d. menschlichen Gehororganes," Stuttgart (Enke), 1889 (see 

 Zeit.f. wiss. Mik.,vn, 3, 1890, p. 364). The reviewer (HAUG) 

 here calls attention to the admirable qualities of phloroglucin 

 as a decalcification agent for this object (see 562). 



EICHLER (Abh. d. math.-phys. Cl. d. k. Sachsischen Ges. d. 

 Wiss., Bd. xviii, 1892, p. 311 ; Zeit.f. wiss. Mik., ix, 3, 1893, 

 p. 380). Detailed account of manipulations for injection of 

 blood-vessels of the labyrinth. 



Dr. LINDSAY JOHNSON greatly recommends the Ehrlich- 

 Biondi stain (see 259, 683). 



Nerves. 



688. WETGERT'S Method for Medullated Nerves (Fortschr. d. 

 Med., 1884, pp. 113, 190; 1885, p. 136; Zeit.f. wiss. Mik., 

 1884, pp. 290, 564; 1885, pp. 399, 484). The ordinary 

 methods of staining with haematoxylin depend on the produc- 

 tion of an aluminium lake of haematoxylin. Weigert's method 

 depends on the formation of another lake, a chromium or 

 copper lake. In consequence of the formation of these lakes 

 haematoxylin acquires the property of staining the myelin of 

 nerves in a quite specific way. 



In Weigert's process the formation of these lakes takes 

 place in the tissue itself. The details of the process have 

 been considerably modified, both by other workers and by 

 Weigert himself. I give here the 1885 method, a knowledge 

 of which is necessary to the understanding of the subsequent 

 modifications. It is as follows : The tissues are to be hard- 

 ened in bichromate of potash (the solutions of Muller or 

 Erlicki will do as well, so far as I know). The hardening 

 need only be carried to the point at which the tissues have 



