KULTSCHITZKY'S METHOD. 367 



The sections are then washed in water and differentiated by the method of 

 Pal. 



BBEGLIA (1. c. in last section) mordants sections for ten to fifteen minutes 

 in a mixture of 15 c.c. of 90 per cent, alcohol with 3 to 7 c.c. of saturated 

 aqueous solution of neutral acetate of copper (ARMANNI). They are then 

 brought for five or ten minutes into a mixture of 3 parts of water with 1 

 part of saturated aqueous solution of lithium carbonate. They are then 

 stained for eighteen to twenty-four hours in the extract of logwood solution 

 given in the last section, to which if desired one-fifth to one-third of the 

 lithium carbonate solution may be added. They are then differentiated in 

 Weigert's decolourising solution. Instead of the acetate of copper, you may 

 take a 4 to 7 per cent, solution of sulphate of copper, and mordant therein 

 for twenty minutes ; or carbonate of copper, subacetate of lead, perchloride 

 of iron, or other salts may be used. 



695. VASALE (Rivista sper. di Freniatria, xv, 1889, p. 902; 

 Zeit.f. wiss. Milt., vii, 4, 1891, p. 517) finds that Weigert's 

 process can be considerably abbreviated as follows. He 

 stains first for three to five minutes in the hsematoxylin, then 

 treats the sections for the same length of time with saturated 

 aqueous solution of neutral acetate of copper, in which they 

 become black. They are then washed with water and dif- 

 ferentiated by moving them about in Weigert's decolourising 

 liquid diluted with one-half of water. 



696. Another rapid modification is that of LISSAUEE, de- 

 scribed by Sachs (Centralb.f. Nervenheilk., xv, 1892, p. 330; 

 Zeit.f. wiss. Mik., ix, 3, 1893, p. 391). Sections of material 

 hardened in liquid of Miiller are heated (the mordantage 

 with copper being omitted) in 1 per cent, solution of chromic 

 acid until the solution begins to form bubbles. They are 

 then rinsed with water, and again heated, until bubbles begin 

 to form, in Weigert's hsematoxylin solution. They are then 

 differentiated by Pal's process. The method is said to succeed 

 even with material that has not been very well hardened, and 

 to give good results with brains that have been only a short 

 time in liquid of Miiller. 



697. KULTSCHITZKY (Anat. Anz. } 1889, p. 223, and 1890, p. 

 519 ; Zeit.f. wiss. Mik., vi, 2, 1889, p. 196, and vii, 3, 1890, p. 

 367) has given two forms of his well-known process, of which 

 the following is the later : Specimens are hardened for one 

 to two months in solution of Erlicki, imbedded in celloidin or 

 photoxylin, and cut. Sections are stained for from one to 

 three hours, or as much as twenty-four, in a stain made by 



