NEUROGLIA. 411 



STILLING'S method (Arch. f. mik. Anat., 1880, p. 471 ; Bau. d. nervosen 

 Centralorgane, 1882). 



CABBIEBE'S (Arch.f. mik. Anat., 1877, p. 126). 



SCHIEFFEEDECKEE'S (ibid., 1878, p. 38; also BEHBENS, KOSSEL, und 

 SCHIEFFEEDECKEE, Das Mikroskop, Bd. ii, p. 227). 



FBEEBOBN'S (Amer. Mon. Mic. Journ., 1888, p. 231 ; Journ. Roy. Mic. 

 Soc., 1889, p. 298). 



782. BEVAN LEWIS'S Compression Method for Fresh Tissue (Mon. 

 Mic. Journ., 1876, p. 106 ; Human Brain, p. 146) is as follows : Vertical 

 sections, as thin as possible, are made from a piece of a convolution of 

 brain from which the membranes have been removed. The sections are 

 made by free hand by means of a section-knife flooded with spirit. The 

 sections are got on to a slide and treated with Muller's solution for a few 

 seconds. A cover is then put on and steadily pressed down so as to flatten 

 out the sections into an almost transparent film. The slide is then rinsed in 

 water and placed for thirty to forty seconds in a bath of methylated spirit. 

 It is then removed, one edge of the cover-glass steadied with the finger, the 

 blade of a penknife inserted under the opposite edge, and the cover gently 

 lifted. The section, which remains adherent either to the slide or to the 

 cover, is washed with water, stained in any way that may be desired* 

 dehydrated, and mounted in balsam. 



For staining the cells of the cortex, Lewis prefers a 1 per cent, solution of 

 anilin black. 



He prefers to dehydrate by drying under a bell-glass in the presence of 

 concentrated sulphuric acid. Clear with chloroform in preference to clove 

 oil. Lewis finds that this process results in far less rupture and tearing of 

 nerve-cells and processes than would be imagined ; he considers that " the 

 result is equivalent to the most delicate teasing of tissue, the processes being 

 gradually unravelled from their dense networks, and the structural elements 

 universally displayed to the best advantage." 



v. THANHOFFEE (Zeit.f. wiss Mik., iv, 4, 1887, p. 467) describes a similar 

 process. 



783. Neuroglia (GIEKKE, Arch. f. mik. Anat., 1885, p. 444). 

 Maceration in the usual media, chromic solutions, iodised 

 serum, &c., but especially in the liquid of Landois ( 532). 

 For sections, harden for six to ten weeks in bichromate of 

 ammonia, first of 1*5 per cent., and gradually raised to 3 per 

 cent. Avoid paraffin for imbedding, and imbed in celloidin 

 or cut without imbedding. Stain with ammonia- carmine, or 

 picro-carminate of soda, or alum-carmine, or Heidenhain's 

 haematoxylin. Other details in this valuble paper. 



784. Myelon of Reptilia and Amphibia. Besides the usual methods 

 which will suggest themselves as being applicable to this class of objects, 

 see MASON'S methods, supra, 742 ; SCHMIDT, Zeit. f. wiss. Mik., 1885, 



