420 SOME OTHEIl HISTOLOGICAL METHODS. 



holic solution of Aurantia, dehydrates in absolute alcohol, 

 clears in clove oil, and mounts in balsam. 



WOLTERS (see Zeit. f. wiss. Mik., ix, 3, 1893, p. 360) mor- 

 dants sections for twenty-four hours in a mixture of two parts 

 10 per cent, chloride of vanadium, and eight parts 8 per cent, 

 acetate of aluminium, stains for twenty-four hours in an in- 

 cubator in Kultschizky's haematoxylin, and differentiates in 

 Weigert's ferricyanide mixture, or, after a short treatment 

 with solution of chloride of iron, in water. 



For a somewhat complicated method of MARTIN OTTI'S, with nitrate of silver, 

 which is, I think, of more theoretical than practical interest, see Zeit. f. wiss. 

 Mik., v, 4, 1888, p. 521. 



For the methods of UNNA and TAENZEE with nitrate of rosanilin salts, 

 see Monatsch. f. prdkt. Dermat., vi, 1887 ; or BEHRENS, KOSSEL, u. SCHIEF- 

 FEEDECKER, Das Mikroskop, 1, p. 204. 



796. Bone, Non-decalcified (RANVIER, Traite, p. 297). 

 Ranvier points out certain precautions that it is necessary to 

 take in the preparation of sections of dry bone. In general, 

 the bones furnished by " naturalists," or procured in ana- 

 tomical theatres, contain spots of fatty substance that prevent 

 good preparations from being made. Such spots are formed 

 when bones are allowed to dry before being put into water 

 for maceration ; when a bone is left to dry the fat of the 

 medullary canals infiltrates its substance as fast as its water 

 evaporates. 



Bones should be plunged into water as soon as the sur- 

 . rounding soft parts have been removed, and should be divided 

 into lengths with a saw whilst wet. The medulla should 

 then be driven out from the central canal by means of a jet 

 of water ; spongy bones should be submitted to hydrotomy. 

 This may be done as follows : An epiphysis having been 

 removed, together with a small portion of the diaphysis, a 

 piece of caoutchouc tubing is fixed by ligature on to the 

 cut end of the diaphysis, and the free end of the piece of 

 tubing adapted to a tap through which water flows under 

 pressure. 



As soon as the bones, whether compact or spongy, have 

 been freed from their medullary substance they are put to 

 macerate. The maceration should be continued for several 

 months, the liquid being changed from time to time. As 

 soon as all the soft parts are perfectly destroyed, the bones 



