OTHER METHODS FOB GLANDULAR STRUCTURES. 433 



mucigen is stained black, but the vacuoles are unstained. Since perruthenic 

 acid is very rapidly reduced by organic matter, Ranvier regards this reaction 

 as a proof that the vacuoles do not contain any organic substance, but pro- 

 bably only water and inorganic salts. 



For detailed instructions for the study of goblet cells, see 

 LIST, in Arch. f. mik. Anat., xxvii, 1886, p. 481. 

 See also ante, 246, 247, 252, 255. 



810. Liver. See hereon the important papers of KANVIEK, 

 " Les membranes muqueuses et le syst. glandulaire," in the 

 Journ. de Microgr., ix, x, 1885-6 ; IGACUSCHI, in Arch. f. path. 

 Anat., xcvii, p. 142, or Zeit. f. wiss. Mik., 1885, p. 243 (gold 

 process for study of fibrous networks) ; KUPFFER, Sitzb. Ges. 

 f. Morph., &c., Miinchen, Juli, 1889, or Zeit. f. wiss. Mik., vi f 

 4, 1889, p. 506 (haematoxylin stain for demonstration of ulti- 

 mate bile-ducts, and application of Golgi's silver bichromate 

 method to the same object and to the study of fibrous net- 

 works) ; OPPEL, Anat. Am., v, 1890, p. 143; vi, 1891, p. 165; 

 and Zeit.f. iviss. Mik., vii, 2, 1890, p. 222; viii, 2, 1891, p. 224 

 (also concerning the application of Golgi's process to the 

 above objects). 



811. Other Methods for Glandular Structures. Amongst 

 numerous important papers that cannot be quoted here, see 

 KANVIEE, "Le mccanisme de la secretion," in Journ. de Microgr., 

 x, 1886-7, and the valuable papers of HEIDENHAIN inPfluger's 

 Archiv. 



The peculiar applicability of the Ehrlich-Biondi stain, 259, 

 to this kind of work hardly needs pointing out. 



Indigo, used as in 241 or 242, may also be found very 

 useful. 



For salivary glands see 252. 



28 



