CLEANSING INTESTINE OF LUMBRICQS. 449 



narcotised by means of cocain of 1 to 2 per cent, strength 

 added to the water in a trough containing them. They 

 should be watched under the microscope until the cilia have 

 just ceased to vibrate, and should then be fixed by running 

 strong solution of Flemming down the sides of the trough by 

 means of a pipette. They are left for fifteen minutes, washed 

 in five or six changes of distilled water, and permanently 

 mounted in distilled water containing a trace of solution of 

 Flemming (about 8 drops to the ounce). For minutiaa 

 concerning the different species see the original. 



Annelida. 



840. Cleansing Intestine of Lumbricus (KUKENTHAL, Journ. 

 Roy. Mic. Soc., 1888, p. 1044). Put the animals into a tall 

 glass vessel which has been filled up with bits of moistened 

 blotting-paper. They gradually evacuate the earthy particles 

 from the gut, and fill it instead with paper. 



VOGT and YUNG (Traite d'Anat. Comp. Prat., v) recommend 

 coffee-grounds instead of paper ; paper becomes rather hard 

 when imbedded, whereas coffee-grounds cut fairly well. 



841. Lumbricus may be anaesthetised by putting the animals 

 into water with a few drops of chloroform. PERRIEE has 

 pointed out that it is better not to let the chloroform act 

 directly in solution on the animals, but to put them into 

 water in a shallow dish, set up a watch-glass with chloroform 

 in the corner of it, and cover the whole. In half an hour the 

 worms will be more or less narcotised, and if allowed to 

 remain will die in a state of extension. 



CERFONTAINE (Arch, de Biol., x, 1890, p. 327; Zeit.f. luiss. 

 Mik., viii, 2, 1891, p. 210) much recommends curare, ad- 

 ministered by interstitial injection of a dose of about 2 c.c. of 

 a 1 : 500 solution. The animal should afterwards be put into 

 water, and after a quarter of an hour will be found dead. 



In order to kill Criodrilus lacuum, COLLIN (Zeit. f. wiss. 

 Zool., xlvi, 1888, p. 474) puts the animals into a closed vessel 

 with a little water, and hangs up in it a strip of blotting- 

 paper soaked in chloroform. KUKENTHAL (Die mik. Technik, 

 1885 ; Zeit. f. wiss. Mik., 1886, p. 61) puts Annelids into a glass 

 cylinder filled with water to the height of 10 centimetres, and 

 then pours 70 per cent, alcohol to a depth of 1 to 2 centi- 



29 



